Instrument
Super-resolution optical microscope
DISCIPLINES
ORGANISATIONS
CONDITION
ADDITIONAL INFO
The main parameters of the localization based super-resolution microscope system operates at the Department of Optics and Quantum Electronics and developed by the Advanced Optical Imaging group (AdOptIm: http://titan.physx.u-szeged.hu/~adoptim/) are the following: The system is based on an Nicon Eclipse Ti-E inverse fluorescent frame. The system can operate either in confocal (commercial C2 unit) and dSTORM super-resolution (developed by the research group) modes. The axial position of the sample can be hold via a perfect focus system with a precision of <20nm. The sample can be illuminated via TIRF, HILO and EPI modes and excited at wavelengths of 647nm, 561nm and 405nm. The spatial resolution of the dSTORM system depends on the sample. The position of the fluorescent molecules in fixed cells and slides can be determined typically with a precision of <20nm.